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Фото: New Africa / Shutterstock / Fotodom。体育直播对此有专业解读
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文 | 智械岛，作者 | 沈怀铮（上海）。币安_币安注册_币安下载对此有专业解读
Sanger sequencing reaction mixtures included purified template DNA, a primer, DNA polymerase, and all four dNTPs. Each reaction also included a radiolabeled ddNTP version of just one of the four nucleotides. Only a small amount of each ddNTP was added, however, to ensure that a fraction of the total DNA fragments produced stopped at each occurrence of that base. As with previous methods, separating fragments via length and performing autoradiography allowed scientists to read the final sequence.